Backcrosses are used to incorporate new genes from a donor parent into existing lines (the recurrent parent). Traditionally this process takes 5 to 8 generations. By fingerprinting a pre-selected progeny of the first two to three consecutive backcrosses, datasets are generated which provide a clear picture of the origin of the genetic material (genetic map) of the progeny. These are used to calculate the percentage of recurrent parent genome and enable the breeder to select the individuals with the highest % of recurrent parent and the desired number of donor parts for breeding.
Its Value
AFLP® based acceleration of inbred conversions:
is an effective tool to increase the speed and quality of backcross conversions;
allows clear visualization of the genotypes of all chromosomes of all individuals;
enables the selection of individuals with approximately 98% genetic material of the recurrent parent in two generations;
gives detailed knowledge regarding the genetic location of the trait of interest;
provides an informative genetic map;
is also applicable for the introgression of QTL-regions and / or removal of linkage drag;
includes co-dominant scoring of hetero and homozygotic markers;
can be used for the development of an integrated genetic map.
Example
The attached KeyFact shows an example of accelerated inbred conversions in maize.
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