The development of SSR-marker based PCR-assays requires a considerable investment. With the assistance of the AFLP® technology the costs and the turnaround time can be reduced considerably.
Its Value
AFLP ® based microsatellite discovery :
can be used as a highly informative fingerprinting technology;
requires no sequence information;
is a powerful tool for random highly polymorphic SSR discovery;
results in substantial reductions of the development costs for SSR based PCR assays;
allows co-dominant scoring of all markers;
allows development of SSR markers with a known information content in the germplasm.
ExamplES
The attached poster illustrates the power of the microsatellite discovery approach:
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