The KeyPoint ™ Technology is Efficient and high through-put mutation screening technology that can be applied on mutagenized populations or to screen for natural occurring variation in targeted gene sequences. In one KeyPoint experiment thousands of samples can be screened simultaneously in a cost-effective manner.
The main features of the KeyPoint technology are:
Massive parallel sequencing approach using the Roche/454 Life Sciences Genome Sequencer System (GS system)
A DNA pooling strategy of DNA samples
Tagged amplification of target gene DNA
Nature of identified variations/mutations (frame shift, stopcodon, AA change or nonsense mutation) directly available
Compared with currently available mutant screening technologies the KeyPoint technology provides additional speed and accuracy in pointing the mutations present in a mutant population.
How it works
Selection of samples to be screened
The Keypoint technology is applied on a starting population of mutagenized samples (in figure ems treated plants) or a set of DNA samples derived from microorganisms, plants, animals or patients to be screened for natural occurring variations in the DNA
Isolation of DNA and DNA samples are pooled
PCR analysis using tagged primers
Sequence analysis
Sequence results deconvoluted and linked to individual samples
technologies 
