KeyPoint™ mutation screen

Applications

• Screening of mutant populations
• e.g. ems populations used for tilling
• Screening for natural variation
• Identified mutations directly applicable for commercial applications in breeding programs

KeyPoint services

• Customized set-up of pooled DNA libraries – in collaboration with customer
• Amplification strategy target genes – including primer design
• High-throughput and ultra deep sequence analysis on pooled samples
• Verification of a mutant seed by Sanger sequencing

Deliverables

• Total number of reads
• Average read length in bp
• Total amount of target sequences generated
• Number of tagged reads
• Number of reads per individual pool coordinate (tag)
• Average number of reads per sample
• Number of variations/mutations identified in samples
• Mutation detection rate
• List of identified variations/mutations identified
• List of samples with identified variations/mutations