With the cDNA-AFLP transcript profiling technology, up to 90% of all expressed genes, whether abundant or rare, can be detected. This allows the identification of genes that are only expressed in pre-defined conditions or cell types. The corresponding upstream promoter and downstream terminator regions are then obtained by long-range linker PCR directly from genomic DNA or from BAC libraries.
Its Value
cDNA-AFLP based promoter identification has the following benefits;;
the promoters will have expression profiles (in the target species) that match exactly customer specifications, whether strong or weak, constitutive or highly specific;
the promoters will be of endogenous origin;
the transcript profiling will lead to a number of candidate promoters and, in an intelligent experimental set-up, to different candidate promoters with different well-defined specificities.
Example
The attached outline shows the experimental set-up of a typical promoter identification project: Promoter Identification Outline.pdf (78 Kb)
An example of a promoter isolated by KeyGene is the strong and constitutive AA6 tomato promoter described in the attached KeyFact : KF Tomato Promoter.pdf (94 Kb)
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