SNP Discovery - CRoPS™ technology

KeyGene offers a variety of techniques for the discovery and validation of Single Nucleotide Polymorphisms (SNPs).

How it works
A discovery set of individuals/lines/species is selected and their DNA isolated. KeyGene then uses five techniques for SNP discovery:

1. CRoPS™ technology is applied to sequence DNA which generates >20Mb of data and visualize the polymorphisms by sequence alignment
2. SFP technology is applied by hybridization of genomic DNA on gene expression chips
3. AFLP ® fingerprints of discovery samples are generated. Polymorphic bands are subsequently sequenced to determine the exact SNP’s;
4. alternatively, genetically mapped AFLP ® markers are identified and linker PCR is applied to identify the SNP causing the AFLP ® polymorphism;
5. fragments of proprietary or public sequences are re-sequenced in the discovery samples and using both public and proprietary EST’s electronic SNP mining is carried out using KeyGene’s in-house SNP discovery software. This software will determine the allele frequency and, as such, the value of the SNP.