Long read sequencing for genome insights

KeyGene & Nanopore: precise, reliable and useful genome information about crops and pathogens

In crop innovation, exploiting genomic information is the key to success. Plant breeders and plant scientists who use genomic information can be faster and better than their competitors when it comes to the development of new successful crop varieties.

 

KeyGene is the perfect genome insights partner for plant breeders and plant scientists around the globe because of our unrivalled capabilities in DNA isolation, the Oxford Nanopore Technology platforms we use, our powerful bioinformatics team and infrastructure and our wide range of research expertise. Thanks to the integrated approach we offer through our crop innovation platforms, we allow our partners to compose their own ‘menu’ of genomic research, bringing them genome insight for a strong market position.

 

Thanks to using PromethION, MinION, and other platforms of Oxford Nanopore Technology, we offer true and usable genome insight for any crop or plant of interest.

Alexander Wittenberg
NGS expert in agriculture

 

KeyGene’s great performance in long read sequencing
  • unique capabilities in high quality uHMW DNA extraction  from plant material
  • expertise in vegetables, field crops, ornamental crops and pathogens
  • more than twelve year experience in long read technology and crop genome assembly
  • tailored analysis solutions
  • fast TAT: 2-6 months

Choose extra information about long read sequencing at KeyGene

Learn more

Nanopore webinar
Genome insights enabling crop innovation using PromethION

Presented by Alexander Wittenberg next generation sequencing expert at KeyGene

Watch video

Nanopore’s white paper
Closing the gap in plant genomes

About the successful whole genome sequencing of two lettuce breeding lines at KeyGene

 

Download white paper


Press release
KeyGene as first PrometION provider worldwide

 

24 may 2018

 

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Presentation
PromethION sequencing of
complex plant genomes

Keynote presentation by KeyGene’s
Alexander Wittenberg at London
Calling, 24-25 May 2018

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Fast, faster, better !

When it comes to whole genome sequencing, Nanopore platforms like GridION and PromethION allow for a vast production of data. Where speed of sequencing is sometimes considered priority, at KeyGene our drive is to deliver useful data and information to our partners: real genome insight.

 

That is why KeyGene developed high molecular weight DNA-isolation protocols yielding unrivaled DNA quality and bioinformatics tools that converts the precise and reliable sequence data form Nanopore sequencing into real genome insight.

Expertise in field crops, ornamental crops, vegetables, pathogens

Sequencing high molecular weight DNA: unique perspectives

After sequencing, the ‘chromosome puzzle’ needs to be made. The larger the puzzle pieces, the faster the puzzle is made. And the better the resulting puzzle will be.

 

KeyGene is known for its unrivaled capacities and track record in producing high-quality plant DNA isolates with large DNA molecules. Putting it in numbers: in spring 2018 we were already able to routinely produce isolates with a read N50 of ≥30 Mb.

 

Where earlier next generation sequencing platforms can only handle smaller DNA molecules, Nanopore technology allows for ultra-high molecular weight DNA fragments, resulting in ultra-long reads and easier genome assembly.

Expertise in field crops, ornamental crops, vegetables, pathogens

Read about the successes of our partners

 

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Independence from public genome data

So far plant breeders and plant scientists generally use public genome references not representing their own commercial varieties or breeding material..

Now is the time to generate your own high-quality genomic reference of your lines and plants of interest using KeyGene’s expertise and capabilities in DNA isolation and bioinformatics in combination with the PromethION platform. This will bring a big leap in your research to study and improve your traits of interest.