Duplex Sequencing with Covalently Closed DNA Ends
Method for increasing the accuracy of sequencing by covalently linking both strands of duplex DNA templates prior to sequencing
Sequence based genotyping (SBG, GBS)
Genotyping SNP, SSR and indel polymorphisms in a single streamlined process for multiple samples.
KeyPoint® Breeding
Multiplex detection of variation of genes / loci of interest, based on high throughput sequencing.
SNPSelect
Genotyping, based upon locus & allele identifiers; a way to amplify and process DNA into probes
Oligonucleotide-delivery to protoplasts
Method to effectively deliver mutagenic nucleobases to plant protoplasts using polyethylene glycol
Combinatorial barcodes
At least two nucleotide sequence barcodes, for identifying sample origin in NGS.
Targeted translocation
Translocation between 2 homologous/homeologous chromosomes via double-strand break and rejoining
Glycerol-free transfection of CRISPR complex
Glycerol free genome editing of protoplasts using CRISPR-CAS protein and in vitro transcribed gRNA
Plant genome editing by Cpf1
Genome editing of plant protoplasts for high mutagenesis frequencies
Genome editing by programmed deaminases
Genome editing using cysteine or adenine deaminase fused to a site-specific nuclease e.g. a CAS
Accurately amplifying mixtures of probes
Production of oligonucleotides, in particular targeting oligonucleotides or nucleic acid probes.
Genome editing for altered protein function
Editing of a coding sequence by two site-specific nucleases that causes a frameshift
Mad7 for genome editing in plant cells
Highly effective Type V CRISPR system for plant cell genome editing
Dual guide for CRISPR editing of plant cells
Effective genome editing CRISPR system nuclease guided by non-covalently linked, crRNA and tracrRNA
Targeted Sequencing
Using gRNA-CAS complexes to selectively sequence target regions
Semi-solid state for nucleic acid handling
For methods and kits for HMW nucleic acid library preparation, effectively reducing shear stress.
Covalently closing DNA strands by TelN for NGS library preparation
TelN adapters / primers for NGS library preparation for amplification free enrichment of target sequences.
Targeted Sequence Addition
Versatile method for the labelling of a target nucleic acid fragment for targeted sequence library preparation
Target enrichment through selective sequencing
A combination of TarSeq and subsequent selective sequencing, a highly effective method for target sequence enrichment.
RNA Transfection in Plant Cells with Modified RNA
Optimized RNA chemistry for increased retention in plant cells, involving modified uridines
Double Decapitation of Plants
Improved protocol using decapitation, for producing plant shoots with genetic modifications through vector-induced targeted genome editing