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KeyGene reference: P111 & P134

Invention

The invention relates to methods and kits for nucleic acid sample preparation for sequencing. In particular, the invention provides the (use of) a combination of at least two nucleotide sequence barcodes, indicated as combinatorial sequence barcodes or split barcodes, for identifying sample origin in high-throughput sequencing.

Benefit

Making use of unique combinations of barcodes, provided to sequence templates by adapter ligation and/or primer amplification, dramatically increases multiplex level options with the same number of required barcodes. In addition, a specific format of the invention allows for single-read double tagging, resulting in a reduction of sequence costs by 50%.

Vision

The present patent family, together with the KeyGene’s patent family on Sequence based genotyping (SBG/GBS)KeyPoint® Breeding, KeyGene® SNPSelect, revolutionize the discovery, detection and use of polymorphisms.

Patent

Click on the link for an overview of the patent family in the European Patent Register: WO2011/155833

Please note that the European Patent Register may not be extensive and/or accurate, for which KeyGene is not responsible. Please contact your patent expert for further information.

Contact

Would you like to explore the possibilities for obtaining a license for this technology? You can contact our licensing team by mail. Would you like to know more about our technologies and licensing possibilities? Visit our webpage about Licensing KeyGene’s proprietary technologies.