Double decapitation of plants for shoot regeneration
Improved protocol using capitation, for regeneration of plant shoots with genetic modifications through vector-induced targeted genome editing
KeyGene reference: P238
Invention
The invented methodology involves a double decapitation process for plants after vector-induced genome editing resulting in shoots with increased edit frequencies. After exposure of the plant to a (viral) vector comprising the programmed nuclease, the shoot apical meristem of the plant is removed by decapitation. Subsequently, a shoot is regenerated at a second location in the plant, which comprises one or more cells having a genetic modification. This protocol ensures the production of a shoot with increased frequencies of targeted genetic modifications.
Vision
This technology addresses the advantages of efficient genetic modification in plant breeding. Thus, by enabling shoot regeneration, for shoots with specific genetic modifications, it supports advancements in crop improvement and genetic research.
Benefits
- Enables precise genetic modifications in plants.
- Facilitates the production of genetically modified shoots without the need for extensive tissue culture techniques
Patent
Click on the link for an overview of the patent family in the European Patent Register: WO2023111225
Please note that the European Patent Register may not be extensive and/or accurate, for which KeyGene is not responsible. Please contact your patent expert for further information.