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The invention entails combining steps of complexity reduction with the introduction of identifiers through ligation or amplification and next-generation sequencing, which allows for highly efficient multiplexed genotyping. Apart from steps of library preparation, the patents cover proprietary elements such as a barcoded 3’-T-overhang adapter and library preparation kits for multiplex genetic variation detection comprising ligation adapters and amplification primers.


The patented technology is the first of its kind to genotype SNP, SSR and indel polymorphisms in a single streamlined process for multiple samples using SBG. Prior knowledge of a reference genome sequence is not required.


Together with the patent family on KeyPoint®, KeyGene’s global SBG portfolio revolutionized the discovery and detection of polymorphisms.

The various workflows, kits, and proprietary elements covered by the patents have wide application in medical and agricultural research and diagnostics, ranging from scoring Single Nucleotide Polymorphism (SNP) markers linked to valuable traits in crops and animals to detection of specific mutations associated with human diseases. Examples of such assay methods are described in Truong et al.1, and Poecke et al.2, including related methods such as GBS3, two-enzyme GBS4, tunable GBS5, RAD6, ddRAD7, 2b-RAD8, SLAF-seq9, and hybrid-capture based NGS10.

Contact us

Would you like to explore the possibilities for obtaining a license for Variation detection by Next Generation Sequencing? You can contact our licensing team by mail.

Would you like to know more about our technologies and licensing possibilities? Visit our webpage about Licensing KeyGene’s proprietary technologies.

Licensed service providers

Beaverton (OR), United States

Middlesex, United Kingdom

Genomics Center, Minneapolis (MN), United States

Biotechnoloy Center, Madison (WI), United States

Shirley (NY), United States

Single-fee license for SBG use in research

KeyGene understands the value of Sequence Based Genotyping for research. We very much welcome the use in research. Therefore we have created an easy, low-cost and transparent solution for non-profit research organizations to easily access KeyGene’s Sequence-Based Genotyping technology for internal research.

Are you interested in a research or commercial license, please contact our licensing team.

Some of our licensees

Commercial in-house use

  • Enza Zaden
  • Rijk Zwaan
  • Limagrain Vegetable Seeds
  • Takii & Co. Ltd.
  • A global seed company

Academic Internal Research, Licensees Europe

  • The James Hutton Institute
  • University of Hohenheim
  • University of Zürich
  • Forschungszentrum Jülich
  • Wageningen University & Research
  • ETH Zürich
  • CNAG-CRG, Barcelona
  • University of Liverpool
  • WSL Swiss Federal Research Institute
  • Aberystwyth University
  • Julius Kühn-Institut
  • INRA
  • Rzeszow University of Technology
  • University of Exeter

Academic Internal Research, Licensees United States

  • University of Minnesota Genomics Center
  • South Dakota State University
  • Oregon State University
  • Tennessee Technological University
  • Samuel Roberts Noble Foundation
  • San Diego Zoo Global
  • North Carolina State University
  • University of Delaware
  • Kansas State University
  • Virginia Tech
  • University of North Carolina at Chapel Hill
  • Utah State University
  • Mississippi State University
  • West-Virginia State University


Click on the links below for an overview of the patent families in the European Patent Register:

Please note that the European Patent Register may not be extensive and/or accurate, for which KeyGene is not responsible. Please contact your patent expert for further information.

Scientific papers describing the technology