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Invention

The invention provides for a versatile method for the labelling of a target nucleic acid fragment for use in protocols for targeted sequence library preparation, making use of prime editing. Making use of guided-CAS complexes and reverse transcriptase, any desired adapter design can be attached to sequence library fragments.

Benefits

The present invention allows for the labeling of selected target sequences with any desired domain, including e.g. indexes and sequence primer binding sites. Amongst others, domains allowing for covalently closing the ends of the target fragments allow for use of the invention in protocols for PCR-free enrichment methods for NGS. PCR-free NGS library preparation methods are not biased by preferential amplification of for instance specific alleles and allow for the detection of epigenetic modifications such as DNA methylation.

Vision

In research and fields like crop breeding, there is a high demand for versatile technologies that can be used e.g. in enrichment of samples to be sequenced for target sequences in a cost-effective way without PCR bias. KeyGene’s targeted sequence addition can be used for targeted sequencing, which is highly instrumental in elucidating complex genomic structures and epigenetic modifications.

Patent

Click on the link for an overview of the patent family in the European Patent Register: WO2022/074058

Please note that the European Patent Register may not be extensive and/or accurate, for which KeyGene is not responsible. Please contact your patent expert for further information.

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