29 June 2023

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Breakthrough shared: Telomere-to-telomere crop genomes using only one DNA sequencing platform

A collaboration between KeyGene scientists and the Telomere to Telomere consortium, which is studying the human genome, has resulted in telomere-to-telomere genome assemblies of tomato and maize, using one sequencing technology only. This shows that generation genome insights to improve plant breeding is now getting really ‘easy’. The breakthrough has been shared at different international podia, one of which can now be (re)viewed online.

From 2 to 1 sequencing platform

In March 2022, the complete sequence of a human genome was published. This milestone achievement was made possible by combining long, accurate PacBio HiFi reads with ultra-long Oxford Nanopore reads in combination with advancements in computational algorithms. This recipe for “telomere-to-telomere” (T2T) genome assembly relies on sequencing data from two different platforms, limiting its adoption. Recently, Oxford Nanopore released an end-to-end workflow for telomere-to-telomere human genome sequencing using only the Oxford Nanopore PromethION device.