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The invention provides for a method to effectively deliver mutagenic nucleobases to plant cell protoplasts using polyethylene glycol (PEG).


Genome editing technologies are rapidly emerging. Many targeted genome editing technologies require the (co-)transfection of single stranded oligonucleotides designed to direct the repair of a mutation of interest. The patented technology ensures optimal survival efficiencies of oligonucleotide transfected protoplasts, thereby increasing the chance of regenerating plants from edited protoplasts.


Efficient targeted genome editing technologies are important research tools in gene validation and may be used for introduction of new traits in crop germplasm. By improving survival of edited protoplast the present invention contributes thereto.


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